METHOD
•Collected water samples from 15+ fountains and taps
•Cultured samples on agar plates to assess microbial growth
•Identified organisms using colony morphology and biochemical markers
•Screened samples for antibiotic resistance genes
KEY FINDINGS
•>70% of fountains contained opportunistic microbes (Candida, Morganella, Serratia)
•>60% of samples tested positive for antibiotic resistance genes (VanM, SUL1)
•Fountains showed higher microbial load than taps at the same locations.
These results indicate that drinking fountains act as microbial accumulation points within building plumbing systems, likely due to stagnation and biofilm formation.
The presence of antibiotic resistance genes suggests a potential pathway for resistance exposure in public environments, which is not addressed by conventional fountain filtration.
Because public fountains are used repeatedly by large populations, even low-level contamination may pose cumulative exposure risks, particularly for immunocompromised individuals.

